Capto s impact12/3/2023 Results from both setups showed similar yield and purity as what can be expected from a traditional setup performed in batch runs. Capto S ImpAct is a BioProcess chromatography medium developed for large-scale purification of biopharmaceutical proteins. Table 1.The active groups of Capto S, Capto Q and Capto DEAE. The second Capto adhere step was performed in FT mode in both setups. 2 Properties of Capto S, Capto Q and Capto DEAE For ion exchange chromatography, Capto S uses a sulfonate group, Capto Q uses a quartenary amine group and Capto DEAE uses a diethylaminoethyl group, as shown in the table below. Two setups were evaluated, where the first Capto S ImpAct polishing step was run in either bind-elute (B/E) or flow-through (FT) mode. Capto™ S ImpAct and Capto adhere media in serially connected columns with an intermediate in-line conditioning step were used. The capture step was followed by two polishing steps in an STP setup on the ÄKTA pure chromatography system. ![]() Praesto SP45 resin was obtained from Purolite (King of Prussia, PA, USA). Using the 3C PCC setup, the capacity utilization could be increased by 56% as compared with an equivalent batch run. MabSelect SuRe LX resin, Capto S ImpAct resin, Tricorn 5/150 column (0.5 cm I.D.) and HiScale 26/40 column (26 cm I.D.) were obtained from Cytiva (Uppsala, Sweden). The results showed consistent yield and purity over time. ![]() To assess robustness of the setup, 10 cycles were performed. HiScreen Capto S ImpAct columns provide fast, reproducible and easy separations in a convenient format. MAb capture was performed using MabSelect SuRe™ LX protein A chromatography medium (resin) in a three-column PCC (3C PCC) setup on the ÄKTA™ pcc 75 chromatography system. HiScreen Capto S ImpAct (4.7 ml) are prepacked columns for small scale purifications, as well as optimization of methods and parameters, such as sample load and binding conditions. In this study, periodic counter current (PCC) chromatography and straight-through processing (STP) technologies were evaluated in a continuous three-step monoclonal antibody (MAb) purification process.
0 Comments
Leave a Reply.AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |